Latex laboratory-gloves: an unexpected pitfall in amphibian toxicity assays with tadpoles

This study examined the unexpected toxic effects of protective latex labora tory gloves on developing amphibians. Mortality after exposure to rinsing w ater from the outside of the gloves was observed in Xenopus laevis and Rana temporaria, with R. temporaria being more sensitive. This phenomenon was f urther confirmed using the microtiter-version of the Microtox-Assay, an in vitro assay for general toxicity. Latex gloves from the specific brand used in the experiment, in which the toxicity to tadpoles was observed for the first time, showed the highest toxicity of all materials and brands tested. Due to the high responsiveness of amphibian tadpoles to latex-glove contam inated rinsing water, special care is necessary when cleaning aquaria durin g toxicological experiments with amphibians as otherwise results may be bia sed. (C) 2001 Elsevier Science B.V. All rights reserved.