Characterisation of the Roundup Ready soybean insert

In this article we describe the isolation and characterisation of the junct ion between insert DNA and plant DNA in the transgenic Roundup Ready soybea n line event 40-3-2. Our results establish that during integration of the i nsert DNA several rearrangements occurred at the 3' NOS junction and that t he genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the character isation of junction regions is, in our opinion, the method of choice to sup port method development for detection and identification of plant biotechno logy-derived products.