Morphological and biochemical characteristics of cholangiocellular carcinoma RS-1 after subcutaneous and intrahepatic transplantation

Kinetics, histological and biochemical characteristics of transplantable ch olangiocellular RS-1 carcinoma were studied after intrahepatic and subcutan eous grafting to outbred rats. Both grafts demonstrated similar growth patt erns, i.e. slow growth with a 10 day latency and an exponential growth phas e at days 18-24 following inoculation. Tumor grow-th rate was 10-fold lower and life span of tumor-bearing rats was longer after intrahepatic transpla ntation as compared to subcutaneous tumors. The tumors had identical histol ogy (solid cancer with mucous areas). Cells in both tumors were characteriz ed by the low mitotic activity, moderate rates of spontaneous apoptosis and necrosis. Biochemical characteristics in case of both routes of grafting w ere also identical, constitutive and benze(a)pyrene-inducible activities of glutathione-S-transferase, NAD(P)H-quinone oxidoreductase and aldehyde deh ydrogenase in tumors being 17-fold lower than in the liver. NAD(P)H-quinone oxidoreductase and aldehyde dehydrogenase in hepatic tumors demonstrated t he greatest fall in activity. RS-1 by the intrahepatic transplantation can be considered as a tumor growth model for regional administration of antica ncer cytostatics.