Starch was extracted from 10 hull-less barley (HB) genotypes [waxy (CDC Can
dle, CDC Alamo, SB 94912, and SB 94917), normal amylose (Phoenix, CDC Dawn,
SR 93102, and SB 94860), and high amylose (SB 94893 and SB 94897)]. Starch
content ranged from 56 to 65%. The purity of the isolated starches was gre
ater than 96%. Average starch yield and extraction efficiency were 44 and 7
1%, respectively. The starches from all genotypes consisted of a mixture of
large lenticular and small irregularly shaped granules. The granules of mo
st starches were intact, whereas in others (SB 94917, SR 93102, and SB 9486
0) they were compound (clustered), The proportions of small (diameter less
than or equal to 10 mum) and large granules (diameter > 10 mum), by total n
umber and by total weight differed among genotypes. Bound lipid content was
positively correlated (r=0.92, P < 0.01) with total amylose content. Free
and bound lipid contents ranged from 0.1-0.3% and 0.3-1.7%, respectively. T
he apparent and total amylose contents ranged from 0-39% and 0-45%, respect
ively. The amounts of amylose complexed with native lipids (total amylose-a
pparent amylose) ranged from 0.5 to 7.8%. The proportion of small granules
was correlated with total amylose content (r=0.59, P < 0.1). However, the a
verage granule diameter was negatively correlated (r=-0.65, P < 0.05) with
total amylose content. The debranched amylopectins of all starches exhibite
d the highest peak in the MALDI-MS spectrum at DP 12. The average chain len
gth (CL) and degree of branching ranged from 17.6-22.7% and 4.4-5.5%, respe
ctively. The short (DP 5-17) and long (DP greater than or equal to 35) chai
ns ranged from 58.2-59.1% and 3.0-12.8%, respectively. The study showed tha
t amylose/amylopectin ratio and amylopectin branch chain length have high c
orrelation with granule size and size distribution in this set of barley ge
notypes. (C) 2001 Elsevier Science Ltd. All rights reserved.