Identification of a mutant locus by noncomplementation of a transposon insertion library in Saccharomyces cerevisiae

Authors
Wiatrowski, HA Carlson, M
Citation
Ha. Wiatrowski et M. Carlson, Identification of a mutant locus by noncomplementation of a transposon insertion library in Saccharomyces cerevisiae, GENETICS, 158(4), 2001, pp. 1825-1827
Citations number
9
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENETICS
ISSN journal
00166731 → ACNP
Volume
158
Issue
4
Year of publication
2001
Pages
1825 - 1827
Database
ISI
SICI code
0016-6731(200108)158:4<1825:IOAMLB>2.0.ZU;2-P
Abstract
We describe a new approach for identifying the gene corresponding to a muta tion in Saccharomyces cerevisiae. A library of mTn-lacZ/LEU2 insertions is tested for failure to complement the mutation, and the noncomplementing ins ertion is used to obtain sequence. This approach offers an alternative to c loning by complementation with a plasmid library.