Objective. The aim of this study was to evaluate additional error in estima
ting red cell loss during abdominal hysterectomy.
Methods. Eighty patients admitted consecutively for abdominal hysterectomy
were recruited. The surgeries were done after heparinizing the suction tubi
ng system to prevent clotting and reducing the vacuum pressure to reduce re
d cell lysis. At the end of the surgery, hematocrit was measured and compar
ed with the patient's venous blood and the blood from the suction container
. The Mann-Whitney test evaluated statistical significance.
Results. Eight patients were excluded for having a hemolyzed blood sample,
receiving a blood transfusion, and having incomplete data. The study cohort
consisted of 72 patients: 54 had a simple hysterectomy and 18 had a radica
l hysterectomy with pelvic and periaortic lymphadenectomy. The hematocrit (
mean +/- standard deviation) in the suction container (19.8 +/- 8.8%) was l
ower than the hematocrit from the venous blood sample (32.4 +/- 6%) (P < 0.
001). The hematocrit in the suction container decreased as the duration of
the surgeries increased. Although the volume of blood in the suction contai
ner was used to estimate blood loss, the concentration of red cells in the
container was consistently lower than those in the venous blood sample. The
magnitude of dilution increased as the length and radical nature of the su
rgery increased.
Conclusions. These findings suggest that other fluid, probably lymph, contr
ibutes to the dilution of red cells in the container and increases the esti
mated blood volume loss during surgery. Estimation of red cell surgical blo
od loss becomes less accurate as the length and radical nature of the surge
ry increase. (C) 2001 Academic Press.