Characterization of the secreted MPT53 antigen of Mycobacterium tuberculosis

Authors
Johnson, S Brusasca, P Lyashchenko, K Spencer, JS Wiker, HG Bifani, P Shashkina, E Kreiswirth, B Harboe, M Schluger, N Gomez, M Gennaro, ML
Citation
S. Johnson et al., Characterization of the secreted MPT53 antigen of Mycobacterium tuberculosis, INFEC IMMUN, 69(9), 2001, pp. 5936-5939
Citations number
20
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
69
Issue
9
Year of publication
2001
Pages
5936 - 5939
Database
ISI
SICI code
0019-9567(200109)69:9<5936:COTSMA>2.0.ZU;2-6
Abstract
MPT53 is a secreted protein of Mycobacterium tuberculosis. Southern transfe r and hybridization showed mpt53 to be conserved in the M. tuberculosis com plex and to have homology with DNA from Mycobacterium avium and other nontu berculous mycobacteria. However, anti-MPT53 polyclonal antibodies detected no antigen in the culture filtrates of M. avium and other nontuberculous my cobacteria. MPT53 of M. tuberculosis induced strong, tuberculosis-specific antibody responses in guinea pigs but induced no delayed-type hypersensitiv ity. Involvement in immune responses during human tuberculosis was very mod est.