Isolation and HPLC quantitative analysis of flavonoid glycosides from Brazilian beverages (Maytenus ilicifolia and M-aquifolium)

Aqueous infusions of Brazilian Maytenus leaves are used as beverages, foods tuffs, and phytomedicines. Previously, we isolated two new flavonoid tetras accharides from the infusion of Maytenus aquifolium leaves that showed anti ulcer activity. In this investigation a new flavonoid tetrasaccharide, kaem pferol-3-O-alpha -L-rhamnopyranosyl (1-6)-O-[alpha -L-arabinopyranosyl (1 - ->3)-O-alpha -L-rhamnopyranosyl (1-2)]-O-beta -D-galactopyranoside (3), was isolated, together with kaempferol tri- and disaccharides and quercetin tr isaccharides from the aqueous infusion of Maytenus ilicifolia leaves. All s tructures were elucidated by ES-MS and NMR spectroscopic methods. The quant itative analysis of the flavonoid glycosides from Maytenus ilicifolia and M . aquifolium has been performed by HPLC.