Y. Mita et al., Mechanical fluid flow enhances High-Affinity-IgE-Receptor-Mediated secretion by mast cells adherent to fibronectin, J ASTHMA, 38(5), 2001, pp. 435-441
We demonstrated the effect of interaction between mechanical fluid flow and
the extracellular matrix on high-affinity-IgE-receptor (Fc epsilon RI)-med
iated secretion by mast cells (cell line MC/9). Mechanical fluid flow did n
ot affect secretion by the cells in the absence of exogenou agonists. Mecha
nical fluid flow did not affect secretion by cells adherent to fibronectin
in the absence of exogenous agonists, but it did enhance Fc epsilon RI-medi
ated secretion by cells adherent to fibronectin. These finding indicate tha
t fluid-flow-induced enhancement of secretion by mast cells requires both e
xogenous agonists and interaction between integrins and the extracellular m
atrix. The present study is the first to demonstrate that mechanical fluid
flow enhances Fc epsilon RI-mediated secretion by a mast cell line adherent
to fibronectin.