Lp. Ford et al., Telomerase can inhibit the recombination-based pathway of telomere maintenance in human cells, J BIOL CHEM, 276(34), 2001, pp. 32198-32203
Telomere length can be maintained by telomerase or by a recombination-based
pathway. Because individual telomeres in cells using the recombination-bas
ed pathway of telomere maintenance appear to periodically become extremely
short, cells using this pathway to maintain telomeres may be faced with a c
ontinuous state of crisis. We expressed telomerase in a human cell line tha
t uses the recombination-based pathway of telomere maintenance to test whet
her telomerase would prevent telomeres from becoming critically short and e
xamine the effects that this might have on the recombination-based pathway
of telomere maintenance. In these. cells, telomerase maintains the length o
f the shortest telomeres. In some cases, the long heterogeneous telomeres a
re completely lost, and the cells now permanently contain short telomeres a
fter only 40 population doublings. This corresponds to a telomere reduction
rate of 500 base pairs/population doubling, a rate that is much faster tha
n expected for normal telomere shortening but is consistent with the rapid
telomere deletion events observed in cells using the recombination-based. p
athway of telomere maintenance (Murnane, J. P., Sabatier, L., Marder, B. A.
, and Morgan, W. F. (1994) EMBO J. 13, 4953-4962). We also observed reducti
ons in the fraction of cells containing alternative lengthening of telomere
-associated promyelocytic leukemia bodies and extrachromosomal telomere rep
eats; however, no alterations in the rate of sister chromatid exchange were
observed. Our results demonstrate that human cells using the recombination
-based pathway of telomere maintenance retain factors required for telomera
se to maintain telomeres and that once the telomerase-based pathway of telo
mere length regulation is engaged, recombination-based elongation of telome
res can be functionally inhibited.