Prostaglandin E-2 regulates the level and stability of cyclooxygenase-2 mRNA through activation of p38 mitogen-activated protein kinase in interleukin-1 beta-treated human synovial fibroblasts

The p38 MAPK mediates transcriptional and posttranscriptional control of cy clooxygenase-2 (COX-2) mRNA following interleukin-1(IL-1)/lipopolysaccharid e cellular activation. We explored a positive feedback, prostaglandin E-2 ( PGE(2))-dependent stabilization of COX-2 mRNA mediated by the p38 MAPK casc ade in IL-1 beta -stimulated human synovial fibroblasts. We observed a rapi d (5 min), massive (> 30-fold), and sustained (> 48 h) increase in COX-2 mR NA, protein, and PGE, release following a recombinant human (rh) IL-1 beta signal that was inhibited by NS-398, a COX-2 inhibitor, and SB202190, a sel ective, cell-permeable p38 MAPK inhibitor. PGE(2) completely reversed NS-39 8-mediated inhibition but not SB202190-dependent inhibition. The eicosanoid didn't potentiate IL-1 beta -induced COX-2 expression nor did it activate COX-2 gene expression in quiescent cells. Transfection experiments with a h uman COX-2 promoter construct revealed a minor element of p38 MAPK-dependen t transcriptional control after IL-1 beta stimulation. p38 MAPK synergized with the cAMP/cAMP-dependent protein kinase cascade to transactivate the CO X-2 promoter. When human synovial fibroblasts were activated with rhIL-1 be ta for 3-4 h (steady state) followed by washout, the elevated levels of COX -2 mRNA declined rapidly (<2 h) to control levels. If PGE(2), unlike EP2/3 agonists butaprost and sulprostone, was added to fresh medium, COX-2 mRNA l evels remained elevated for up to 16 h. SB202190 or anti-PGE(2) monoclonal antibody compromised the stabilization of COX-2 mRNA by PGE2. Deletion anal ysis using transfected chimeric luciferase-COX-2 mRNA 3 ' -untranslated reg ion reporter constructs revealed that IL-1 beta increased reporter gene MIR NA stability and translation via AU-containing distal regions of the untran slated region. This response was mediated entirely by a PGE(2)/p38 MAPK-dep endent process. We conclude that the magnitude and duration of the induct-i on of COX-2 mRNA, protein, and PGE(2) release by rhIL-1 beta is primarily t he result of PGE(2) -dependent stabilization of COX-2 mRNA and stimulation of translation, a process involving a positive feedback loop mediated by th e EP4 receptor and the downstream kinases p38 MAPK and, perhaps, cAMP-depen dent protein kinase.