S. Van Huffel et al., Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2, J BIOL CHEM, 276(32), 2001, pp. 30216-30223
The nuclear factor kappaB (NF-kappaB) plays a central role in the regulatio
n of genes implicated immune responses, inflammatory processes, and apoptot
ic cell death. The zinc finger protein A20 is a cellular inhibitor of NF-ka
ppaB activation by various stimuli and plays a critical role in terminating
NF-kappaB responses. The underlying mechanism for NF-kappaB inhibition by
A20 is still unknown. A20 has been shown to interact with several proteins
including tumor necrosis factor (TNF) receptor-associated factors 2 and 6,
as well as the inhibitory protein of kappaB kinase (IKK) gamma protein. Her
e we report the cloning and characterization of ABIN-2, a previously unknow
n protein that binds to the COOH-terminal zinc finger domain of A20. NF-kap
paB activation induced by TNF and interleukin-1 is inhibited by overexpress
ion. of ABIN-2. The latter also inhibits NF-kappaB activation induced by ov
erexpression of receptor-interacting protein or TNF receptor-associated fac
tor 2. In contrast, NF-kappaB activation by overexpression of IKK beta or d
irect activators of the IKK complex, such as Tax, cannot be inhibited by AB
IN-2. These results indicate that ABIN-2 interferes with NF-kappaB activati
on upstream of the IKK complex and that it might contribute to the NF-kappa
B-inhibitory function of A20.