Renal vascular resistance properties and glomerular protection in early established SHR hypertension

Objective To characterize the in vivo vascular properties of the spontaneou sly hypertensive rat (SHR) renal vascular bed by examining vascular conduct ance/resistance responsiveness to vasoactive agents in vivo and determining whether the filtration surface area of glomerular capillaries is reduced. Design and methods In vivo renal blood flow responses to intrarenally admin istered angiotensin II, phenylephrine and acetylcholine were compared in 10 -week-old SHR and Wistar-Kyoto (WKY) rats using a wide range of doses from near threshold to near maximal effect. Unbiased stereological techniques an d high-resolution light microscopy were used to estimate the surface area a nd length of glomerular capillaries, and evidence of capillary damage. Results The SHR renal bed demonstrated significantly enhanced dose-vascular resistance responses to vasoconstrictors. For vascular conductance and cal culated radius of resistance vessels, the SHR curves were significantly low er across the full dilator-constrictor range examined, but the dose-related changes were similar to those of WKY rats. There were only modest enhancem ents of the renal blood flow responses in the SHR, evident only when renal blood flow was reduced by more than 50% SHR and WKY rats did not differ in mean glomerular capillary surface area (0.13 +/- 0.02 mm(2) and 0.14 +/- 0. 02 mm(2), respectively) or length (5.76 +/- 0.85 mm and 5.48 +/- 0.90 mm, r espectively) nor was there evidence of glomerular capillary damage in eithe r strain. Conclusions The renal vascular bed of the SHR in vivo exhibits reduced vasc ular conductance across a wide vasomotor range, compatible with findings in other vascular beds. We have further shown no evidence of reduced glomerul ar capillary surface area or damage. These findings are compatible with the hypothesis that the reduced conductance of the SHR pre-glomerular vasculat ure increases the aorta-capillary pressure gradient thus protecting the glo merular capillaries from systemic hypertension at this age. (C) 2001 Lippin cott Williams & Wilkins.