Phylogenetic relationships among 4 subspecies of Neotoma floridana and sist
er species from the United States and Mexico were examined using DNA sequen
ce data from the mitochondrial DNA cytochrome b gene. Parsimony, likelihood
, and neighbor-joining analyses support the recognition of N. f. magister a
s a distinct species (N. magister). Populations of eastern woodrats from th
e southern and western United States also possessed relatively high levels
of sequence divergence (5.3%), which may be indicative of ongoing divergenc
e between these populations. These findings support conclusions from previo
us studies and add clarity to the biogeographic processes influencing evolu
tion and diversification of this group of rodents.