The MeOH extract of an Indonesia Eudistoma sp. ascidian contained 1,3,O-7-t
rimethylisoxanthopterin (1), a novel pteridine. The purification of I was a
chieved through flash Cis chromatography and cyano HPLC. The structure was
determined primarily through the use of H-1-C-13 and H-1-N-15 HMBC measurem
ents and comparison with data obtained for 1,3,7-trimethylguanine (2).