Rn. Rolfe et al., Analysis of responses of Leidynema appendiculata to acetylcholine using electrophysiological techniques, J PARASITOL, 87(4), 2001, pp. 917-919
The electrophysiological responses of adult females of the insect-parasitic
nematode Leidynema appendiculata, to 100 mM. 10 mM, and 1 mM acetylcholine
were concentration dependent, with stimulation by 100 mM acetylcholine giv
ing the largest increase in spike activity. By contrast. the delay in respo
nse was not concentration dependent. No increase in activity was obtained o
n stimulation with either 0.1 mM acetylcholine or phosphate-buffered saline
. The lack of response to 0.1 mM acetylcholine was confirmed by sequential
exposure of L. appendiculata to 0.1 mM acetylcholine and subsequently to 10
mM acetylcholine.