Molecular recognition in a reconstituted tumor cell membrane

The design of an immunoliposome system for molecular recognition using reco nstituted, hydrogel-supported bilayer lipid membranes (sb-BLMs) is describe d. By monitoring the electrical properties. two kinds of recognition are fe asible: (i) the human bladder tumor cells, Ej and its antibody BDI-1, the l ifetime of the reconstituted membrane is 42 min, and (ii) the human rectum tumor cells, LOVO, the life of the reconstructed membrane is more than 40 m in, the same as conventional BLM. Further, the anticancer drug, Adriamycin (Anticancer Res., 20 (2000) 1391), was shown to be effective in such recons tituted systems, the life of which is less than 5 min. In these experiments , the active ingredients of the Ej and LOVO cells were determined on recons tituted sb-BLMs. The key point is that the component part being recognized on the BLM must be kept in its native state. (C) 2001 Elsevier Science B.V. All rights reserved.