Vanadium uptake and an effect of vanadium treatment on F-18-labeled water movement in a cowpea plant by positron emitting tracer imaging system (PETIS)

We present real time vanadate (V5+) uptake imaging in a cowpea plant by pos itron emitting tracer imaging system (PETIS). Vanadium-48 was produced by b ombardin a Sc foil target with 50 MeV alpha -particles at Takasaki Ion Acce lerators for Advanced Radiation Application (TIARA) AVF cyclotron. Then V-4 8 was added to the culture solution to investigate the V distribution in a cowpea plant. The real time uptake of the V-48 was monitored by PETIS. We m easured the distribution of V-48 in a whole plant after 3, 6 and 20 hours o f V treatment by Bio-imaging Analyzer System (BAS). After the 20 hour treat ment, vanadate was detected at the up-ground part of the plant. To know the effect of V uptake on plant activity, F-18-labeled water uptake was analyz ed by PETIS. When a cowpea plant was treated with V for 20 hours before F-1 8-labeled water uptake experiment, the total amount of F-18-labeled water a bsorption was drastically decreased. Results suggest the inhibition of wate r uptake was mainly caused by the vanadate already moved to the up-ground p art of the plant.