Al behavior in tobacco cells by NAA and staining method

In the study of aluminum toxicity for plant, it is important to analyze the behavior of Al in cells. We present how Al is taken up by tobacco cells th rough lumogallion staining method developed by our laboratory. The fluoresc ence intensity from the cell was increased rapidly between 4 and 8 hours of I mM Al treatment. To calibrate the fluorescence intensity from Al-lumogal lion complex, Al amount in the cell was determined by NAA. When the same sa mple was analyzed by ICP-AF-S, Al amounts in all the samples were 13% lower than those measured by NAA.