ROLE OF 3'-CCA SEQUENCE IN INTERACTION OF TRNA(PHE) FROM ESCHERICHIA-COLI AND THERMUS-THERMOPHILUS WITH HOMOLOGOUS PHENYLALANYL-TRANSFER-RNA-SYNTHETASES

Authors
MOOR NA STEPANOV VG REPKOVA MN VENYAMINOVA AG VRATSKIKH LV YAMKOVOI VI MOTORIN YA LAVRIK OI
Citation
Na. Moor et al., ROLE OF 3'-CCA SEQUENCE IN INTERACTION OF TRNA(PHE) FROM ESCHERICHIA-COLI AND THERMUS-THERMOPHILUS WITH HOMOLOGOUS PHENYLALANYL-TRANSFER-RNA-SYNTHETASES, Biochemistry, 59(9), 1994, pp. 959-962
Citations number
16
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
59
Issue
9
Year of publication
1994
Pages
959 - 962
Database
ISI
SICI code
0006-2979(1994)59:9<959:RO3SII>2.0.ZU;2-#
Abstract
The 3'-CCA terminus in tRNA(Phe) from E. coli and Thermus thermophilus was substituted with other sequences (AAA, CCC, UUU, and UUA) by step wise degradation and subsequent enzymic ligation of shortened RNA with the respective trinucleotides (pA)(3), (pC)(3), and (pU)(3). The kine tic characteristics of aminoacylation by the modified tRNA were invest igated. The 3'-terminal trinucleotide sequence in tRNA(Phe) is importa nt for the recognition, binding, and aminoacylation by phenylalanyl-tR NA-synthetases from E. coil and Thermus thermophilus.