Probing of DNA and single-base mismatches by chemical force microscopy using peptide nucleic acid-modified sensing tips and functionalized surfaces

Authors
Lioubashevski, O Patolsky, F Willner, I
Citation
O. Lioubashevski et al., Probing of DNA and single-base mismatches by chemical force microscopy using peptide nucleic acid-modified sensing tips and functionalized surfaces, LANGMUIR, 17(17), 2001, pp. 5134-5136
Citations number
28
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
LANGMUIR
ISSN journal
07437463 → ACNP
Volume
17
Issue
17
Year of publication
2001
Pages
5134 - 5136
Database
ISI
SICI code
0743-7463(20010821)17:17<5134:PODASM>2.0.ZU;2-O
Abstract
DNA hybridization to peptide nucleic acid (PNA)-functionalized Au-coated ti ps is characterized by probing the force interactions between the PNA-funct ionalized tip or the DNA/PNA-functionalized tip with a hydrophobic 1-mercap toundecane-modified Au surface, using chemical force microscopy (CFM). DNA concentrations as low as 3 x 10(-13) M can be detected by probing the respe ctive force interactions. The CFM method is sufficiently sensitive to detec t a single base mismatch,in the analyte DNA.