Establishment of CD7(+) human myeloma sister cell lines, KMS-21-PE and KMS-21-BM, carrying t(11;14) and t(8;14)

Two new human myeloma cell lines were established from pleural effusion and bone marrow malignant cells derived from a single patient, who manifested hyperammonemia associated with multiple myeloma, and these were characteriz ed. Both lines possess t(11;14)(q13;q32) and t(8;14)(q24;q32) reciprocal tr anslocations and overexpress cyclin DI, but not c-myc. Human myeloma lines including these new lines produced and secreted excess ammonia into culture medium more than non-myelomatous hematological cell lines. In addition, th ese two lines were revealed to have high surface CD7 expression correlated with relatively high mRNA expression by MP-RT-PCR. Among 8 human myeloma li nes, half of them revealed significant surface expression of CD7 and a posi tive correlation between expression levels of protein and message. CD7 mess age was also detected in surface negative lines. Consequently, there may be posttranslational regulation of the CD7 molecule, whose cellular biologica l role in expressing cells has not been elucidated.