Effects of mineral nutrients on the growth of bacterio- and phytoplankton in two southern reservoirs

Nutrient limitation of bacterio- and phytoplankton was studied simultaneous ly in two warm-water lakes in the southern United States-Joe Pool Lake JPL) and Eagle Mountain Lake (EML). Lakes were sampled approximately biweekly b etween March 1998 and December 1999 from a single station. Nutrient limitat ion was assessed through dilution bioassays in which nitrogen (N, 50 muM ab ove ambient), phosphorus (P, 10 muM above ambient), carbon (C, bacterioplan kton only, 83 muM above ambient), and trace nutrients (Tm, phytoplankton on ly) were supplied. In both lakes, growth of bacterio- and phytoplankton was stimulated by nutrient additions. Multiple nutrient Limitation was common. P alone and in combination with N and C or Tm most frequently limited grow th of both bacterio and phytoplankton in JPL. N alone and in combination wi th P and C or Tra most frequently Limited growth of both bacterio- and phyt oplankton in EML. Comparison of in situ growth rates to growth rates under potentially nutrient saturating conditions revealed that both bacterio- and phytoplankton in both lakes were growing well below maximum potential duri ng warm months but near maximum potential during cooler months. This result was due to a combined effect of low temperature in winter and restricted n utrient availability in summer. Phytoplankton was generally more strongly l imited by nutrient availability than was bacterioplankton, but there were o ccasions when the intensity of limitation shifted between communities.