LIPOXYGENASE PATHWAY OF OXIDATION OF ARACHIDONIC-ACID IN MOUSE SPLENOCYTES AND ITS MODULATION BY GM(3) GANGLIOSIDE LACTONE

Authors
BEZUGLOV VV FOMINAAGEEVA EV TEKIEVA EA DYATLOVITSKAYA EV
Citation
Vv. Bezuglov et al., LIPOXYGENASE PATHWAY OF OXIDATION OF ARACHIDONIC-ACID IN MOUSE SPLENOCYTES AND ITS MODULATION BY GM(3) GANGLIOSIDE LACTONE, Biochemistry, 59(9), 1994, pp. 1007-1012
Citations number
21
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
59
Issue
9
Year of publication
1994
Pages
1007 - 1012
Database
ISI
SICI code
0006-2979(1994)59:9<1007:LPOOOA>2.0.ZU;2-6
Abstract
Major arachidonic acid metabolites released by mouse splenocytes into the incubation medium were identified by chromatography and spectrosco py and by mass spectrometry of derivatives of the metabolites. With or without exogenous arachidonic acid, splenocytes mainly produced 12-hy droxy-5,8,10,14- and 12,20-dihydroxy-5,8,10,14-eicosatetraenoic acids. Both products (the latter predominating) were continuously released b y intact cells without exogenous substrate or modulators. This is the first demonstration that exogenous ganglioside GM(3) and its lactone m odulate the metabolism of arachidonic acid in splenocytes.