Fluorimetric determination of amphetamine in urine by flow injection with on-line liquid-liquid extraction

Determination of amphetamine in urine was performed by batch and flow injec tion methodologies. The suitable experimental conditions for fluorimetric m easurements were established. The liquid-liquid extraction was carried out at pH 13 using diethyl ether as extracting reagent. The measurement conditi ons were 260 nm and 277 nm for excitation and emission wavelengths, respect ively. The method requires standard addition calibration and Youden blank c orrection. The influence of the main metabolites of amphetamine and metamph etamine were studied. The accuracy and precision of the proposed method was tested and the method is adapted to the flow injection procedure with on-l ine extraction. (C) 2001 Elsevier Science B.V. All rights reserved.