Profile of phosphoprotein labelling in organotypic slice cultures of rat hippocampus

In recent years organotypic slice cultures of hippocampal tissue of rats ha ve been widely used to study factors involved in neuronal death. Here we us ed 2D electrophoresis to study the phosphoprotein profile in such cultures and the effect of oxygen/glucose deprivation on this profile. Cultures were prepared from 7-day-old rats. After 14 days in culture the phosphorylation profile in the cultures, as shown by phosphoprotein markers undergoing dev elopmental change, closely resembled the profile of fresh tissue from 23-da y-old rats. The results suggest that this model could be a good method to o bserve the development of the tissue and its response to an ischaemic lesio n NeuroReport 12:2705-2709 (C) 2001 Lippincott Williams & Wilkins.