Ha. Peredo et Sm. Celuch, Bradykinin and electrical stimulation increase prostaglandin production inthe rat vas deferens, PROS LEUK E, 65(1), 2001, pp. 9-14
Citations number
31
Categorie Soggetti
Cell & Developmental Biology
Journal title
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
The epididymal portion of the rat vas deferens produced prostaglandins (PG)
E-2, F-2 alpha and 6-keto F-1 alpha, Electrical stimulation (ES, 0.1 Hz,1
ms) increased such production by 100%, and similar results were obtained in
the presence of 1.0 muM bradykinin (Bk). When both stimuli were applied si
multaneously, the increases in PG production were 1100% for PGE(2), 800% fo
r PGF(2 alpha) and 400% for PG6-keto F-1 alpha. Prazosin abolished the eff
ect of ES on PG production. A selective Bk B-2-receptor antagonist abolishe
d the increase in PG production induced by Bk, both in non-stimulated and i
n ES tissues. Bk (1.0 muM) elicited contractile responses in non-stimulated
as well as in ES tissues, responses that were not modified in the presence
of 10 muM indomethacin. In conclusion, the effects of Bkon prostaglandin p
roduction appears to depend on the activation of B-2 receptors, while the i
ncrease in prostaglandin release induced by ES, and the effects observed wi
th both stimuli simultaneously, should be mediated by the release of noradr
enaline and the subsequent activation of alpha (1) adrenoceptors. (C) 2001
Harcourt Publishers Ltd.