Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites. II. Identification and quantification of 19-norsteroids responsible for 19-norandrosterone and 19-noretiocholanolone excretion in human urine
K. De Wasch et al., Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites. II. Identification and quantification of 19-norsteroids responsible for 19-norandrosterone and 19-noretiocholanolone excretion in human urine, RAP C MASS, 15(16), 2001, pp. 1442-1447
In previous work (Le Bizec et al., Rapid Commun. Mass Spectrom. 2000; 14:10
58), it was demonstrated that a boar meal intake could lead to possible fal
se accusations of abuse of 17 beta -nortestosterone in antidoping control.
The aim of the present study was to identify and quantify endogenous 19-nor
steroids in boar edible tissue at concentrations that can alter the steroid
urinary profile in humans, and lead to excretion of 19-norandrosterone (19
-NA) and 19-noretiocholanolone (19-NE). The samples were analysed in two la
boratories. The methodologies used for extraction and detection (GC/MS(EI)
and LC/MS/MS(APCI+)) are compared and discussed. 19-Norandrostenedione (NAE
D), 17 beta- and 17 alpha -nortestosterone (bNT, aNT), and 17 beta- and 17
alpha -testosterone (bT, aT) were quantified. The largest concentrations of
NAED and bNT were observed in testicles (83 and 172 mug/kg), liver (17 and
63 mug/kg) and kidney (45 and 38 mug/kg). A correlation between the bNT an
d NAED content of a typical meal prepared with boar parts and the excreted
concentrations of 19-NA and 19-NE in human urine was demonstrated. Copyrigh
t (C) 2001 John Wiley & Sons, Ltd.