Stereoselective reduction of alkyl 3-oxobutanoate by carbonyl reductase from Candida magnoliae

Authors
Yasohara, Y Kizaki, N Hasegawa, J Wada, M Kataoka, M Shimizu, S
Citation
Y. Yasohara et al., Stereoselective reduction of alkyl 3-oxobutanoate by carbonyl reductase from Candida magnoliae, TETRAHEDR-A, 12(12), 2001, pp. 1713-1718
Citations number
35
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON-ASYMMETRY
ISSN journal
09574166 → ACNP
Volume
12
Issue
12
Year of publication
2001
Pages
1713 - 1718
Database
ISI
SICI code
0957-4166(20010716)12:12<1713:SROA3B>2.0.ZU;2-Y
Abstract
The enantioselective reduction of alkyl 3-oxobutanoates by carbonyl reducta se (Sl) from Candida magnoliae was investigated. S1 reduced alkyl 4-halo-3- oxobutanoates to the corresponding enantiomerically pure (S)-3-hydroxy este rs. Escherichia coli HB101 transformant co-overproducing the S1 and glucose dehydrogenase from Bacillus megaterium, produced optically pure alkyl 4-su bstituted-3-hydroxybutanoates in a two-phase water/organic solvent system. (C) 2001 Elsevier Science Ltd. All rights reserved.