Dominant genetic markers such as AFLPs and RAPDs are usually analyzed based
on the presence or absence of a band on an electrophoretic gel. This type
of analysis does not allow a distinction among dominant homozygotes and het
erozygotes. Such a distinction is possible based on the quantitative measur
ement of band intensities. In the present paper, we consider the problem of
analyzing dominant markers based on band-intensity data. The basic step fo
r mapping a marker is to assess its recombination frequency with other mark
ers. Ordering markers on a map can then be done using a number of standard
procedures. For this reason estimation of the recombination frequency is th
e main focus of the present paper. The method is demonstrated for the case
of an F-2 population. By simulation we investigate its accuracy and compare
it to the standard estimation based on dominant scoring for band presence/
absence. There are a number of potential applications. For example, the map
may be used to locate quantitative trait loci (QTLs), applying standard pr
ocedures modified to account for uncertainty of the marker genotype. Moreov
er, map information can be used to determine the most likely genotype at a
marker, given its band intensity and the band intensities at flanking marke
rs.