YEAST MITOCHONDRIAL F1F0-ATPASE - THE NOVEL SUBUNIT-E IS IDENTICAL TIM11

We report here the identification of the novel subunit of the mitochon drial F1F0-ATPase from Saccharomyces cerevisiae, ATPase subunit e. Yea st ATPase subunit e displays significant similarities in both amino ac id sequence, properties (hydropathy and predicted coiled-coil structur e) and orientation in the inner membrane,,vith previously identified m ammalian ATPase subunit e proteins, Estimation of its native molecular mass and ability to be co-immunoprecipitated with a subunit of the F- 1-ATPase, demonstrate that subunit e is a subunit of the F1F0-ATPase. Stable expression of subunit e requires the presence of the mitochondr ially encoded subunits of the F-0-ATPase, Subunit e had been previousl y identified as Tim11 and was proposed to be involved in the process o f sorting of proteins to the mitochondrial inner membrane. (C) 1997 Fe deration of European Biochemical Societies.