GENOMIC ORGANIZATION AND FUNCTIONAL-CHARACTERIZATION OF THE MOUSE GALR1 GALANIN RECEPTOR

Galanin mediates diverse physiological functions in digestive, endocri ne, and central nervous systems through G-protein-coupled receptors, T wo galanin receptors have been cloned but the gene structures are unkn own. We report genomic and cDNA cloning of the mouse GalR1 galanin rec eptor and demonstrate that the coding sequence is uniquely divided int o three exons encoding the N-terminal portion through the fifth transm ebrane domain, the third intracellular loop, and the sixth transmembra ne domain through the C-terminus, Functional analysis of the encoded c DNA revealed active ligand binding and intracellular signaling, The ex pression is detected in brain, spinal cord, heart and skeletal muscle. (C) 1997 Federation of European Biochemical Societies.