Epididymal epithelial cell involution following a single intraperitoneal administration of ethane dimethanesulfonate in the goat (Capra hircus)

Ethane dimethanesuifonate (EDS) selectively destroys Leydig cells in rats a nd a few other smaller animal species but not in mice and quail. In the tel eost fish, it stimulates testicular activity instead. It also causes format ion of sperm granulomas, reduction of sperm fertilizing ability, and destru ction of clear cells in the epididymis. Investigations involving larger ani mal species are scanty. We have previously reported that EDS has no effect on goat Leydig cells but appears to have a direct cytotoxic effect on the s eminiferous epithelium. This study was therefore designed to investigate th e effects of EDS on goat epididymal cytoarchitecture. EDS was administered intraperitoneally at two dose levels, 75 and 25 mg/kg body wt. The former d ose was rather toxic, killing three of five goats in this group within 24 h whereas the latter dose was well tolerated. Six days after treatment, the goats were hemicastrated and the epididymis was isolated and processed for light and electron microscopy. Involution associated with EDS was observed in epithelial cells of all regions of the epididymis, each having its own s pecific and peculiar changes. In the caput, there was increased cytoplasmic density accompanied by enlarged vacuoles and paucity of secretory vesicles in the apical cytoplasm. The Golgi cisternae were dilated and disorganized and, in the basal aspect, large dense staining bodies or inclusions, degen erative mitochondria, and lamellated bodies were observed. In the corpus, l arge vacuoles containing flocculent materials occurred in the entire cell c ytoplasm but were particularly numerous and large in the midcytoplasm, comp letely obliterating the Golgi area. There was a dramatic reduction in epith elial height in the cauda epididymis accompanied by sparse distribution of markedly shortened microvilli. The epithelial cells had extensively lobulat ed nuclei and disorganized cytoplasm with dilated Golgi apparatus and large conglomerations of tubular structures. These structural changes suggest th at EDS causes degeneration of goat epididymal epithelial cells. These effec ts are likely to result from the direct action of the compound on the epith elium. (C) 2001 Academic Press.