A. Cuellar et al., FURTHER-STUDIES ON THE RECOUPLING EFFECT OF 6-KETOCHOLESTANOL UPON OXIDATIVE-PHOSPHORYLATION IN UNCOUPLED LIVER-MITOCHONDRIA, FEBS letters, 411(2-3), 1997, pp. 365-368
The effect of 6-ketocholestanol was studied on CCCP-induced uncoupling
in liver mitochondria, submitochondrial particles and cytochrome oxid
ase proteoliposomes. It was found that 6-ketocholestanol prevents and
reverses uncoupling induced by nM concentrations of CCCP on the three
systems assayed. As it was reported on kidney mitochondrial membranes
[Chavez et al. (1996) FEES Lett. 379, 305-308], the recoupling effect
caused by 6-ketocholestanol on submitochondrial particles and proteoli
posomes could be due to a diminution of membrane fluidity. (C) 1997 Fe
deration of European Biochemical Societies.