Temporal effects of 17 beta-estradiol on caveolin-1 mRNA and protein in bovine aortic endothelial cells

Endothelial nitric oxide synthase (eNOS) is regulated both by caveolin-1 an d 17 beta -estradiol (E-2). Temporal relationships between effects of estro gen on caveolin-1 and nitric oxide (NO) are not known. Therefore, this stud y was designed to determine whether estrogen regulates caveolin-1 and, if s o, whether such regulation corresponds to changes in nitrite/nitrate (NO.) production. Bovine aortic endothelial cells (BAECs) were cultured in the ab sence and presence of 17 beta -estradiol or 17 alpha -estradiol (10(-8) and 10(-10) M) for 12, 24, and 48 h. eNOS protein expression and NO, productio n increased significantly after 24 h but not after 12-h treatment with 17 b eta- and not 17 alpha -estradiol. Both mRNA and protein for caveolin-1 were increased significantly only after 48-h treatment with E2, but eNOS protei n and NO. production were decreased compared with cells treated for 24 h. T hese increases in caveolin-1 were inhibited by the estrogen receptor antago nist ICI-182,780 (10(-6) M). Results of this study suggest that E-2 stimula tes caveolin-1 transcription and translation through estrogen receptor-medi ated mechanisms. The results further suggest that estrogen may indirectly r egulate NOx through caveolin-1 expression, which inhibits eNOS catalytic ac tivity.