Ta. Sasaki et al., Analysis of heterocyclic amines in mainstream cigarette smoke using a new NCI GC-MS technique, ANAL LETTER, 34(10), 2001, pp. 1749-1761
A new procedure for the analysis of heterocyclic amines (HAs) in cigarette
mainstream smoke condensate was developed. The procedure is based on two st
ep derivatization with heptafluorobutyric anhydride (HFBA) and N,N-dimethyl
formamide dimethyl acetal, followed by GC-MS analysis using negative chemic
al ionization (NCI) and single ion monitoring (SIM) detection. Using this p
rocedure 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhiP) and 2-amino
-3-methylimidazo[4,5-f]quinoline (IQ) can be analyzed at levels as low as 0
.5 ng/cig. Besides IQ and PhiP, the presence of six other HAs in particulat
e phase smoke was investigated. The technique is not sensitive enough for t
he analysis of 2-amino-9H-pyrido[2,3-b]indole (A alphaC), 2-amino-3-methyl-
9H-pyrido[2,3-b]indole (MeA alphaC) and 3-amino-1, 4-dimethyl-5H-pyrido[4,3
-b]indole (Trp-P-1). The levels of 3-amino-1-methyl-5H-pyrido[4,3-b]indole(
Trp-P-2), 2-amino-6-methyldipyrido[1,2-alpha :3'2'-d]imidazole (Glu-P-1) an
d 2-aminodipyrido[1,2-alpha :3'2'-d]imidazole (Glu-P-2) were below the esti
mated detection Limit of the procedure.