Simultaneous saccharification and fermentation of amorphous cellulose to ethanol by recombinant Klebsiella oxytoca SZ21 without supplemental cellulase

Authors
Zhou, SD Ingram, LO
Citation
Sd. Zhou et Lo. Ingram, Simultaneous saccharification and fermentation of amorphous cellulose to ethanol by recombinant Klebsiella oxytoca SZ21 without supplemental cellulase, BIOTECH LET, 23(18), 2001, pp. 1455-1462
Citations number
27
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
18
Year of publication
2001
Pages
1455 - 1462
Database
ISI
SICI code
0141-5492(200109)23:18<1455:SSAFOA>2.0.ZU;2-O
Abstract
A derivative of Klebsiella oxytoca M5A1 containing chromosomally integrated genes for ethanol production from Zymomonas mobilis (pdc, adhB) and endogl ucanase genes from Erwinia chrysanthemi (celY, celZ) produced over 20 000 U endoglucanase l(-1) activity during fermentation. In combination with the native ability to metabolize cellobiose and cellotriose, this strain was ab le to ferment amorphous cellulose to ethanol (58-76% of theoretical yield) without the addition of cellulase enzymes from other organisms.