Sequential liquid-liquid extraction of D-amino acid oxidase from Trigonopsis variabilis

Authors
Singh, P Verma, N Marwaha, SS
Citation
P. Singh et al., Sequential liquid-liquid extraction of D-amino acid oxidase from Trigonopsis variabilis, BIOTECH LET, 23(18), 2001, pp. 1479-1483
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
18
Year of publication
2001
Pages
1479 - 1483
Database
ISI
SICI code
0141-5492(200109)23:18<1479:SLEODA>2.0.ZU;2-F
Abstract
A method for isolation of D-amino acid oxidase (DAAO) from disrupted Trigon opsis variabilis cells has been developed. In an aqueous two-phase system c onsisting of PEG6000 (220 g l(-1)), potassium phosphate (110 g l(-1), K2HPO 4 + KH2PO4 = 10.1:1, mol mol(-1)) and dl-methionine (11 g l(-1)), the major portion of cellular proteins (87%) was partitioned into the salt phase. By sequential extraction, 48% of DAAO was recovered in PEG phase, giving a yi eld of 211 U mg protein(-1).