Immobilization of lipase by salts and the transesterification activity in hexane

Lipases from six different sources were immobilized on Celite and five type s of salt. The transesterification activities in hexane for lipases immobil ized on EDTA-Na-2 increased by 463% for the lipase from Candida rugosa (CRL ), 2700% for the lipase from Candida sp. (CSL) and 1215% for the lipase fro m Pseudomonas sp. (PSL), compared to the salt-free enzyme. With 0.5% sucros e for CRL or 1% sorbitol for PSL as the lyoprotectant during lyophilization process, transesterification activity increased by 100% and 13%, respectiv ely, compared to the immobilized enzyme on EDTA-Na-2 without lyoprotectant.