Partial genetic characterization of West Nile Virus strains, New York State, 2000

We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN ) virus strains collected in New York State during the 2000 transmission se ason to determine whether they differed genetically from each other and fro m the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic di stances were 0.003 or less. WN virus strains circulating in 2000 were homog eneous with respect to one another and to a strain isolated in 1999.