Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay

Authors
Johnson, DJ Ostlund, EN Pedersen, DD Schmitt, BJ
Citation
Dj. Johnson et al., Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay, EM INFECT D, 7(4), 2001, pp. 739-741
Citations number
6
Categorie Soggetti
Clinical Immunolgy & Infectious Disease
Journal title
EMERGING INFECTIOUS DISEASES
ISSN journal
10806040 → ACNP
Volume
7
Issue
4
Year of publication
2001
Pages
739 - 741
Database
ISI
SICI code
1080-6040(200107/08)7:4<739:DONAWN>2.0.ZU;2-G
Abstract
A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avia n tissue and infected cell cultures. However, the procedure lacks the sensi tivity to detect WN virus in equine tissue. We describe an RT-nested PCR (R T-nPCR) procedure that identifies the North American strain of WN virus dir ectly in equine and avian tissues.