Influence of dynamic conditions on biofilm formation by staphylococci

The modified microtiter plate test was used to investigate biofilm formatio n by staphylococci under both static and dynamic conditions. The quantity o f biofilm produced under static conditions was used as a reference. Dynamic conditions, which were achieved by incubating microtiter plates on a horiz ontal shaker with and without the presence of glass beads in wells, either reduced biofilm formation or left it unchanged. Dynamic conditions particul arly affected the capacity of certain species to produce biofilm: these spe cies included the causative agents of infections associated with a foreign body (Staphylococcus epidermidis, Staphylococcus aureus). On the basis of t hese results, dynamic conditions should be included as a parameter for eval uating biofilm formation by staphylococci in vitro.