PCR-RFLP typing detects new HLA-DRB1 alleles: DRB1*13022, DRB1*1336 and DRBI*1435

It is difficult to resolve all heterozygous combinations of the HLA-DRB1*03 , *08, *11, *12, *13 and *14 allele group in a one-step generic HLA-DRB1 ty ping system. Therefore, it is common to employ a secondary technique utiliz ing group-specific primers to amplify this group of alleles separately from the other HLA-DRB1, -DRB3, -DRB4 and -DRB5 alleles. This paper describes a polymerase chain reaction-restriction fragment length polymorphism (PCR-RF LP) method for broad typing of the HLA-DRB1*03, *08, *11, *12, *13 and *14 alleles which, as well as being time-efficient and cost-effective, has so f ar allowed the detection of 10 new alleles. The new alleles were identified after following up unusual or novel PCR-RFLP patterns. Of the 10 novel all eles found so far with this method, seven have been described previously wh ile three, DRB1*13022, DRB1*1336 and DRB1*1435, are presented here.