Sequence polymorphism in the coding region of mitochondrial genome encompassing position 8389-8865

Analysis of the polymorphic sequences in mitochondrial DNA (mtDNA) has been widely applied to forensic tests and anthropology studies. However. these polymorphic data in human have thus far been derived from the displacement- loop and intergenic regions only. Here. we report the identification of clu stered polymorphic sites in the mitochondria coding region encompassing pos ition 8389-8865. The DNA sequences of 119 unrelated Chinese were determined by PCR amplification and direct sequencing. The results showed that hetero plasmy was found in five individuals, 39 sites were noted in this 477 bp re gion, and 41 haplotypes were identified. The probability of identity and al lelic diversity were estimated as 0.1265 and 0.8809, respectively. The resu lts suggest that sequence polymorphism from position 8389-8865 in human mtD NA can be used as a marker for identity investigation. (C) 2001 Elsevier Sc ience Ireland Ltd. All rights reserved.