We describe the generation of a P1 artificial chromosome genomic library fr
om the Southern pufferfish, Spheroides nephelus. The arrayed library consis
ts of similar to 30,000 clones and has an average insert size of 125-150 kb
. The coverage is estimated to encompass seven to eight genome equivalents.
The library has been used for isolating numerous genomic clones and for es
tablishing contigs of several multigene families. Analysis of several of th
e clones from this library suggests a preponderance of CA re eat tracts rel
ative to their abundance in humans. The library and high-density filters ha
ve been made available to the scientific public through genomics distributi
on companies. (C) 2001 Published by Elsevier Science B.V. All rights reserv
ed.