A modified choline-deficient, ethionine-supplemented diet protocol effectively induces oval cells in mouse liver

Several reliable and reproducible methods are available to induce oval cell s in rat liver. Effective methods often involve inhibiting proliferation in hepatocytes using an alkylating agent, then subjecting the rat to partial hepatectomy (PH). The surgery is difficult to perform reproducibly in mice. Approaches that do not include partial hepatectomy, such as administration of D-galactosamine, are ineffective in mice. We found that a choline-defic ient, ethionine-supplemented (CDE) diet, which is very effective in rats, l eads to high morbidity and mortality when administered to mice. This articl e outlines an alternative protocol by which a CDE diet can be administered to mice. This diet is shown to be highly effective for oval cell induction, without causing high mortality. It takes less time and is at least as effe ctive as other commonly used protocols for inducing oval cells in mice.