Purification and characterization of a 29 kDa poly(A)-binding protein fromchickpea (Cicer arietinum) epicotyl

Authors
Cheriyath, V Balasubrahmanyam, A Kapoor, HC
Citation
V. Cheriyath et al., Purification and characterization of a 29 kDa poly(A)-binding protein fromchickpea (Cicer arietinum) epicotyl, I J BIOCH B, 38(4), 2001, pp. 258-262
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
ISSN journal
03011208 → ACNP
Volume
38
Issue
4
Year of publication
2001
Pages
258 - 262
Database
ISI
SICI code
0301-1208(200108)38:4<258:PACOA2>2.0.ZU;2-W
Abstract
A poly(A)-binding protein (PABP) with mol wt 29,000 has been purified from chickpea (Cicer arietinum) epicotyl by ammonium sulfate fractionation and C ibacron blue F3-GA chromatography, making a complex with poly(A) and elutio n of PABP-poly(A) complex at 45 degreesC from oligo d(T)-cellulose. The elu tion pattern and binding properties show that the purified protein is diffe rent from the PABP (mol. wt 72,000) reported earlier from our laboratory.