Multiple isoforms of protein kinase C in lymphocytes and airway smooth muscle of guinea pig

The isoenzyme pattern of protein kinase C (PKC) in lymphocytes and airway s mooth muscles (ASM) was examined by Western blot using commercially availab le monoclonal antibodies. The results showed the presence of PKC alpha, bet a, gamma, epsilon, eta, mu, xi and in lymphocytes and PKC alpha, gamma, eps ilon, eta and xi in ASM. The unexpected feature was the presence of PKC gam ma in both lymphocytes and ASM of guinea pigs. Expression of this PKC isofo rm is usually restricted to tissues in the central nervous system or spinal cord. Expression of PKC delta, theta, lambda and tau was not detected in e ither lymphocytes or ASM.