Ba. Bohne et Gw. Harding, PROCESSING AND ANALYZING THE MOUSE TEMPORAL BONE TO IDENTIFY GROSS, CELLULAR AND SUBCELLULAR PATHOLOGY, Hearing research, 109(1-2), 1997, pp. 34-45
A technique has been developed for preparing the mouse temporal bone f
or histopathological examination: first, as a whole mount to detect an
y gross malformations of the bony or membranous labyrinths; second, in
dissected segments to localize damage in the different sensory organs
and to quantify sensory- and supporting-cell losses; and finally, in
semi-thick and thin sections to identify and characterize subcellular
pathology. Examples are given of the successful application of this te
chnique to mice with very different inner-ear problems, including thos
e with an abnormally short cochlear spiral, a defective lateral semici
rcular canal, abnormal otoliths over the saccular macula, an increased
susceptibility to noise damage and those which lack fibroblast growth
factor receptor 3.