A packed-bed fungal bioreactor for the continuous decolourisation of azo-dyes (Orange II)

The degradation of an azo dye, Orange II, by immobilised Phanerochaete chry sosporium in a continuous packed bed bioreactor for periods longer than 30 days has been carried out. Nearly complete decolourisation ( > 95%) was ach ieved when working at a high dye load rate of 0.2 g l(-1) d(-1), a temperat ure of 37 degreesC, a hydraulic retention time (HRT) of 24 h and applying o xygen gas in a pulsed flow. These conditions allowed Manganese peroxidase ( MnP) production and the subsequently Orange II decolourisation. A correlati on between residual MnP activity in the effluent and decolourisation was es tablished. Apparently, for decolourisation to be effective, a minimum MnP a ctivity was required, no substantial increase in efficiency at MnP activiti es higher than 10 U l(-1) was observed. The treatment caused, the breakdown of the chromophoric group as well as the cleavage of the aromatic ring. (C ) 2001 Elsevier Science BY. All rights reserved.